Total cAMP PDE Activities in the Presence and Absence of Cilostamide from HASMCs after prolonged exposure to DbcAMP: The effect of inhibitors of mRNA and protein synthesis
| Vehicle | DbcAMP | DbcAMP + ActD | DbcAMP + CHX | |
|---|---|---|---|---|
| Total cAMP PDE activity (pmol/min/mg protein) | 133 ± 7 | 376 ± 382-a | 145 ± 252-b | 142 ± 212-b |
| +1 μM cilostamide (pmol/min/mg protein) | 103 ± 52-a | 235 ± 232-b | 104 ± 122-c | 95 ± 102-d |
cAMP PDE activities are means ± S.E. from triplicate determinations of three independent experiments using 1 μM cAMP as substrate.
↵2-a P < .05 vs. vehicle-treated HASMC total activity.
↵2-b P < .05 vs. DbcAMP (1 mM; 16 h)-treated total cellular activity.
↵2-c P < .05 vs. total PDE activity from cells treated with DbcAMP and actinomycin D (ActD; 4 μM).
↵2-d P < .05 vs. total activity from cells treated with DbcAMP (1 mM) and cycloheximide (CHX; 100 μM).