Stimulation of IP Turnover | Inhibition of Substance P Induced IP Turnover | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ZnCl2 | Substance P | ZnCl2 | Zn2+(phenantroline)3 | Cu2+(phenanthroline)3 | |||||||||
EC50 S.E.M. | EmaxS.E.M. | (n) | EC50S.E.M. | (n) | EmaxS.E.M. | (n) | IC50S.E.M. | (n) | IC50S.E.M. | (n) | IC50 S.E.M. | (n) | |
μM | fmol/105cells | nM | fmol/105cells | μM | μM | μM | |||||||
hNK1-wt | NS | (8) | 0.36 ± 0.1 | (5) | 38 ± 3 | (8) | >1000 | (4) | >1000 | (3) | >1000 | (3) | |
P112H-hNK1 | NS | 0.54 ± 0.1 | (4) | 32 ± 4 | (3) | 550 ± 100 | (3) | >1000 | (3) | >1000 | (3) | ||
P112D-hNK1 | NS | 0.29 ± 0.03 | (4) | 25 ± 8 | (3) | 770 ± 180 | (3) | >1000 | (3) | >1000 | (3) | ||
M291C-hNK1 | NS | 0.55 ± 0.1 | (4) | 26 ± 7 | (4) | 210 ± 73 | (3) | >1000 | (3) | >1000 | (3) | ||
P112H;M291C-hNK1 | 84 ± 19 | 4.1 ± 1.1 | (5) | 1.70 ± 0.5 | (4) | 38 ± 5 | (5) | 39 ± 11 | (3) | 120 ± 12 | (3) | 237 ± 64 | (3) |
P112D;M291C-hNK1 | 83 ± 17 | 5.8 ± 1.5 | (6) | 2.50 ± 0.3 | (4) | 27 ± 2 | (6) | 26 ± 5 | (4) | 190 ± 96 | (3) | 36 ± 10 | (3) |
Stimulatory effect of Zn2+ and Substance P and inhibitory effect of Zn2+, Zn2+(phenantroline)3 and Cu2+(phenanthroline)3 on Substance P (30 nM) induced stimulation. Receptors were transiently expressed in COS-7 cells, and inositol phosphate accumulation was performed as described. Determinations were performed in duplicate and n indicates the number of experiments.
IP, inositol phosphate; NS, no stimulation in IP turnover was observed for 10−4 M and 10−3 M concentrations of ZnCl2.