Regioselective composition of HETEs produced by murine cecum and colon microsomes
| Regioisomer | Distribution | |
|---|---|---|
| Cecum | Colon | |
| % | ||
| 8-HETE | N.D. | 14.8 |
| 9-HETE | 9.2 | 3.2 |
| 11-HETE | N.D. | 16.1 |
| 12-HETE | 20.5 | 3.6 |
| 15-HETE | 3.6 | 6.4 |
| 16-HETE | 8.8 | N.D. |
| 18-HETE | N.D. | 5.1 |
| 19-HETE | 54.7 | 3.3 |
| 20-HETE | 3.2 | 47.5 |
The regiochemical of HETEs formed by incubation of cecum and colon microsomes with [1-14C]AA were quantified by HPLC and liquid scintillation as described under Experimental Procedures.
N.D., nondetectable.