Basal ± S.E. | pEC50 ± S.E. | Emax ± S.E. | |
---|---|---|---|
dpm | % | ||
hEdg2 | 20,099 ± 1,931 | 8.31-a | 101-a |
hEdg2 + wild-type rGiα1 | 25,037 ± 3,984 | 8.0 ± 0.2 | 25 ± 1 |
hEdg2 + rGiα1HA(N) | 23,279 ± 2,085 | 7.7 ± 0.1 | 27 ± 2 |
hEdg2 + rGiα1myc(N) | 30,557 ± 4,352 | 7.6 ± 0.1 | 30 ± 5 |
hEdg2 + mutant rGiα | 19,986 ± 1,851 | 7.6 ± 0.1 | 48 ± 3 |
hEdg2-rGiα1 fusion | 22,693 ± 2,506 | 7.3 ± 0.1 | 69 ± 10 |
No DNA | 12,472 ± 720 | NA | NA |
Levels of basal activity, i.e., in the absence of agonist, seen in membranes prepared from HEK cells transiently transfected with the hEdg2 receptor (alone or together with the various Gi constructs) or the hEdg2 receptor-rGiα1 fusion protein or mock-transfected HEK cells are presented as dpm bound. Potency values for LPA-induced [35S]GTPγS binding carried out in membranes prepared from each condition are given as pEC50 values, and efficacy values (E max) are the maximal stimulation achieved. Results are arithmetic means ± S.E. of four experiments.
↵1-a Only two determinations were made.