Table 1

Cis -inhibition of 10 μM [3H]taurocholate and 0.57 μM [3H]digoxin uptake by GSH and some organic anions in Oatp2-expressing oocytes

InhibitorConcentration[3H]Taurocholate[3H]Digoxin
mM % of control
Control100  ± 28100  ± 24
GSH2095  ± 1886  ± 16
Ouabain112  ± 21-150 14  ± 21-150
DNP-SG166  ± 31-150 62  ± 81-150
BSP0.111  ± 41-150
LTC4 0.5 × 10−3 92  ± 13
BSP-SG0.582  ± 14

Oocytes injected with Oatp2 cRNA (5 ng/oocyte) were incubated with either 10 μM [3H]taurocholate or 0.57 μM [3H]digoxin for 10 min at 25°C. Control uptakes were 127 ± 36 and 193 ± 46 fmol · oocyte−1 · 10 min−1 for [3H]taurocholate and [3H]digoxin, respectively. Values are mean ± S.D. (n = 4–6).

    • 1-150  Significantly different from control,P < .05 by Student's t test.