Substrate | Concentration | H2O | Oatp1 | Oatp2 |
---|---|---|---|---|
μM | fmol · oocyte−1 · h−1 | |||
Taurocholate | 1 | 3 ± 2 | 400 ± 452-150 | 41 ± 152-150 |
Digoxin | 0.57 | 63 ± 8 | 80 ± 4 | 690 ± 1182-150 |
DNP-SG | 50 | 64 ± 2 | 668 ± 1102-150 | 74 ± 10 |
LTC4 | 0.01 | 0.23 ± 0.03 | 0.60 ± 0.222-150 | 0.28 ± 0.06 |
Oocytes were injected with either Oatp1 cRNA (0.5 ng/oocyte), Oatp2 cRNA (5 ng/oocyte), or 50 nl of water. Uptake was measured for 1 h under conditions described in Experimental Procedures. Values are mean ± S.D. of at least three experiments, each performed in quadruplicate.
↵2-150 Significantly different from water-injected oocytes,P < .05 by Student's t test.