Fold activation of different reporter genes by G11αQ209L and GqαQ209L
| CREB | SRE | Elk-1 | |
|---|---|---|---|
| GqαQ209L | 4.8 ± 0.7 | 1.9 ± 0.6 | 1.1 ± 0.6 |
| G11αQ209L | 9.1 ± 1.0 | 3.5 ± 0.2 | 0.9 ± 0.2 |
HEK 293T cells were transfected with constructs directing the expression of G11αQ209L (0.5 μg/plate) or GqαQ209L (0.5 μg/plate) in the presence of CREB-β-galactosidase (0.5 μg/plate), SRE-luciferase (0.5 μg/plate), or pFR-luciferase (0.1 μg/plate) plus pFA2-Elk-1 (0.5 μg/plate). Transfection efficiency was normalized using a cytomegalovirus-β-galactosidase or cytomegalovirus-luciferase control vector. The amount of luciferase or β-galactosidase activity was measured 1 day later. Data are reported as the fold activation ± 2 S.D. of three experiments performed in duplicate compared to the activity of the reporter genes in the absence of activator.