HepG2 cells were detached by treatment with PBS-EDTA, resuspended and incubated for 15 min at 37°C in 200 μl of HEPES-buffered DMEM containing either vehicle (basal), 1 μM UK14304, 10 μM forskolin, or 10 μM forskolin plus 1 μM UK14304. Concentrations of cAMP were measured by radioimmunoassay. Results are expressed as means ± S.E.M. of eight determinations. Statistical analysis was performed using Student's t test.

• ↵2-150  Significant diminution of cAMP level by UK14304 atP < .001.