Addition | cAMP Level |
---|---|
pmol/mg cell protein | |
None | 25 ± 4 |
UK14304 | 29 ± 6 |
Forskolin | 1040 ± 234 |
Forskolin + UK14304 | 547 ± 2342-150 |
HepG2 cells were detached by treatment with PBS-EDTA, resuspended and incubated for 15 min at 37°C in 200 μl of HEPES-buffered DMEM containing either vehicle (basal), 1 μM UK14304, 10 μM forskolin, or 10 μM forskolin plus 1 μM UK14304. Concentrations of cAMP were measured by radioimmunoassay. Results are expressed as means ± S.E.M. of eight determinations. Statistical analysis was performed using Student's t test.
↵2-150 Significant diminution of cAMP level by UK14304 atP < .001.