Ligand | Half-Maximal Reversal of Vanadate(+5)-Evoked Inhibition of Acid Phosphatase RC501-a | Keff/pH 7.2 Values b | Activation of Lipogenesis in Rat Adipocytes |
---|---|---|---|
mM | [103M−1] | ||
EDTA | 0.08 ± 0.03 | 14 | −1-c |
Picolinate | 0.8 ± 0.05 | 1.4 | − |
Maltolate | 0.9 ± 0.07 | 1.24 | − |
l-Glu(γ)HXM | 6.0 ± 0.3 | 0.19 | + + + + 1-c |
l-Asp(β)HXM | 8.0 ± 0.4 | 0.14 | + + + + |
l-Ile(α)HXM | 8.3 ± 0.5 | 0.135 | + 1-c |
l-Arg(α)HXM | 8.4 ± 0.7 | 0.133 | + |
l-Glu | >140 ± 15 | <0.008 | −1-c |
l-Gln | >170 ± 12 | <0.0066 | − |
l-Asn | >140 ± 12 | <0.008 | − |
Citrate | >200 ± 20 | <0.0056 | − |
GSH | >120 ± 10 | <0.0093 | − |
↵1-a Performed as described under Experimental Procedures and in the legend to Fig. 3.
1-b Calculated based on the documented affinity of EDTA to vanadate at neutral pH under similar experimental conditions (Crans et al., 1989).
↵1-c (−), (+), and (++++) refer to ligands that are inactive, partially active, or highly active, respectively, in activating lipogenesis when added at 100 μM concentration to control adipocytes or at 10 μM concentration to vanadium-enriched adipocytes.