Table 1

Primers used for PCR amplification, sequencing, and genotyping

Primer1-aNameSequenceLocation
2A6ex1F1-b 5′-GCT GAA CAC AGA GCA GCT GTA CA-3′Exon 1
2AE3F1-c 5′-GCG TGG TAT TCA GCA ACG GG-3′Exon 3
2A6WTF1-b 5′-CTC ATC GAC GCC CT-3′Exon 3
2Aex7F1-d 5′-GGC CAA CAT GCC CTA CAT G-3′Exon 7
2A6ex8F1-d 5′-CAC TTC CTG AAT GAG-3′Exon 8
2A7ex8F1-d 5′-CAT TTC CTG GAT GAC-3′Exon 8
2A6ex9F5′-CAC CTA AGG ACA TTG ACG TGT CCC-3′Exon 9
2A6In9F5′-AAA AGG AGA TGA CGG CAC AGC-3′Intron 8
2AE3R1-c 5′-TCG TCC TGG GTG TTT TCC TTC-3′Intron 3
2A6ex4R1-b 5′-GGA GGT TGA CGT GAA CTG GAA GA-3′Exon 4
2A6R11-d 5′-GCA CTT ATG TTT TGT GAG ACA TCA GAG ACA A-3′3′ flanking
2A7R15′-GCA CTT ATG TTT TGT GAG ACA TCA GAT AGA G-3′3′ flanking
2A6R21-d 5′-AAA ATG GGC ATG AAC GCC C-3′3′ flanking
2A7R25′-AAA ATG GGC ATG AAC GCT T-3′3′ flanking
2A6S1F5′-GAA GAG TAG TAA TAA TAG CAG-3′3′ flanking
2A6S2F5′-AGG GAC ACA ACG AGA CAT GA-3′3′ flanking
2A6S3F5′-GCA CAA TCC TTG AAA GAA GC-3′3′ flanking

  • ↵1-a  F, forward upstream primer; R, reverse downstream primer.

  • ↵1-b  Primers from Oscarson et al. (1998).

  • ↵1-c  Primers from Fernandez-Salguero et al. (1995).

  • ↵1-d  Primers from Oscarson et al. (1999b).