Second order reaction rates (l/mol-s) of pCMBS− applied in the presence of the indicated reagent with the Cys-substitution mutants
| No GABA | Saturating GABA | 100 nM Diazepam | 100 nM Flumazenil | |
|---|---|---|---|---|
| A291C | 353 ± 162 (3) | 274 ± 118 (3) | N.D. | N.D. |
| Y294C | 44 ± 4 (3) | 153 ± 35 (4) | 50 ± 8 (3) | 53 ± 6 (3) |
| F296C | — | N.D. | 47 ± 22 (3) | N.D. |
| F298C | — | 36 ± 7 (4) | 41 ± 12 (4) | 46 ± 11 (3) |
| L301C | — | 29 ± 4 (3) | 61 ± 15 (3) | 28 ± 9 (3) |
| E303C | — | 198 ± 58 (5) | — | — |
Data given as means ± S.E.M. (n).
N.D., not determined; —, no reaction.