Table 1

Binding affinities (K_i) of disintegrins and RGD peptides on integrins as assessed by SDS-PAGE and autoradiography

	α₈β₁	α_3/5/vβ₁	α_vβ₃
Echistatin^1-a	1.8 ± 0.5	4.2 ± 2.7	1.7 ± 0.5
Elegantin^1-a	0.05 ± 0.12	0.22 ± 0.16	0.18 ± 0.21
Flavostatin^1-a	0.05 ± 0.19	0.22 ± 0.46	0.18 ± 0.22
Flavoridin^1-a	1.4 ± 0.6	4.2 ± 1.4	0.06 ± 0.22
acPenRGDC^1-b	47 ± 136	2.6 ± 1.3	0.008 ± 0.001
cycloRGDdFV^1-b	4.3 ± 0.8	4.1 ± 0.2	0.06 ± 0.01
GRGDdSP^1-b	480 ± 483	270 ± 353	4.3 ± 1.9

Partial amino acid sequence (one-letter symbol) of the peptides used:
`Echistatin:.....KEGTICKRARGDDMDDYCNGK......`
`Elegantin:......–KR–––R–––––NP––R–T–Q......`
`Flavostatin:....–KR–––RI––––FP––R–T–L......`
`Flavoridin:.....–KR–––R–––––NP––R–T––......`
`acPen–––C`
`cyclo–––dFV`
`G–––dSP`

Dissociation constants were calculated by the Cheng-Prusoff equation from the EC₅₀ estimated by Hill analysis assuming that¹²⁵I-echistatin has a concentration of 1.25 nM and binds with an affinity of 1.24 nM (Fig. 1).

↵1-a Results in nM.
↵1-b Results in μM.