Metabolism of cycloSal-[methyl-3H] d4TMP (R p and S p diasteromers) in CEM cells in function of time
| Time | d4T +Prodrug (fr 2–3)2-a | d4T-MP (fr 9–10–11)2-a | d4T-DP (fr 15–16)2-a | d4T-TP (fr 25–26)2-a |
|---|---|---|---|---|
| h | pmol/109 cells | |||
| cycloSal-[methyl-3H]d4TMP (RP diastereomer) | ||||
| 6 | 64 | 234 | 16 | 334 |
| 24 | 56 | 91 | 7.6 | 151 |
| 48 | 74 | 29 | 7.1 | 61 |
| cycloSal-[methyl-3H]d4TMP (Spdiastereomer) | ||||
| 6 | 31 | 29 | 1.5 | 49 |
| 24 | 45 | 16 | 1.8 | 36 |
| 48 | 65 | 10 | 2.8 | 32 |
| cycloSal-[methyl-3H]d4TMP (Rp +Sp diastereomers) | ||||
| 6 | 46 | 146 | 8.2 | 181 |
| 24 | 79 | 49 | 5.1 | 101 |
| 48 | 68 | 20 | 5.5 | 46 |
Data are the means for two independent experiments.
↵2-a Elution times (min) (or fraction numbers) of the metabolites.