Metabolites of 1 μM cycloSal-[methyl-3H] d4TMP in CEM, MT-4, Molt 4/C8, DAUDI, PBL , and M/M cells after 24 h of incubation
| Cell Line | d4T+ Prodrug (fr 2–3)4-a | d4T-MP (fr 9–10–11)4-a | d4T-DP (fr 15–16)4-a | d4T-TP (fr 25–26)4-a |
|---|---|---|---|---|
| pmol/109 cells | ||||
| CEM | 85 | 93 | 19 | 296 |
| MT-4 | 37 | 41 | 17 | 207 |
| MOLT 4/C8 | 60 | 74 | 24 | 472 |
| DAUDI | 40 | 78 | 19 | 585 |
| PBL | 2.7 | 1.1 | ND4-b | 0.2 |
| PBL (activated) | 16 | 8.8 | 0.2 | 5.9 |
| M/M | 15 | 21 | 8.8 | 79 |
Data are the means for two independent experiments (except for the data obtained in CEM cell cultures, which were the means for four independent experiments). The metabolic studies were performed with the slow-eluting R p diastereomer of cycloSal-d4TMP.