Construct | Expression |
---|---|
% | |
Wild-type | 100 |
Deletions | |
ΔNH3 (68–93) | 78 ± 5 |
ΔEC3 (339–344) | 91 ± 17 |
Point mutants | |
I88A | 90 ± 14 |
S89A | 93 ± 16 |
L96A | 100 ± 6 |
EC2DE (E241A, D256A, E260A, E264A) | 83 ± 10 |
D256A | 101 ± 7 |
D256N | NT |
E260A | NT |
E347A | 92 ± 12 |
E347Q | 102 ± 10 |
C175S | 57 ± 6 |
C175S, C254S | 70 ± 9 |
COS-7 cells transiently expressing the wild-type or mutant receptors were incubated with thrombin for 10 min at 37°C. Expression levels were detected in fixed cells with a PAR-1 antibody as described. The expression levels are presented as a percentage of wild-type PAR-1 (100%). Data presented are mean of three independent experiments.
NT, not tested.