Table 1

Flow cytometric analysis of MKN-74 cells synchronized with nocodazole (0.1 μg/ml) for 12 h (Noc₁₂), or nocodazole for 12 h followed by either drug-free media for 24 h (Noc₁₂→(−)₂₄) or nocodazole for 12 h followed by huanglian for 24 h (Noc₁₂→HL₂₄). The cells were harvested at indicated time points and 20,000 gated events were analyzed for DNA content of each sample. Fixed cells were labeled with primary MPM-2 monoclonal antibody followed by secondary goat anti-mouse-fluorescein isothiocyanate antibody to determine the M phase fraction. Data are representative of three independent experiments.