Characterization of CCK binding to CHO-CCKR cells and cell lines coexpressing the receptor intracellular domain peptides
| Cell lines | CCK BindingKi | CCK Receptors Bmax |
|---|---|---|
| nM | × 105 sites/cell | |
| CHO-CCKR | 6.6 ± 0.7 | 1.2 ± 0.3 |
| CHO-CCKR + 1i | 8.8 ± 1.0 | 0.5 ± 0.1 |
| CHO-CCKR + 2i | 10.3 ± 1.4 | 1.0 ± 0.3 |
| CHO-CCKR +3iN | 3.1 ± 0.4 | 1.3 ± 0.4 |
| CHO-CCKR + 3iC | 6.7 ± 0.3 | 1.6 ± 0.5 |
| CHO-CCKR + 4i | 5.3 ± 1.6 | 0.9 ± 0.3 |
| CHO-CCKR + 4i409–423 | 6.2 ± 0.7 | 1.5 ± 0.5 |
Data represent means ± S.E.M. for a minimum of three independent experiments.