Effects of various agents on the level of cGMP remaining in cells 2 or 5 min after removal of NO with Hb
| Compound | Net residual cGMP | |
|---|---|---|
| 2 min after Hb | 5 min after Hb | |
| pmol/106 cells | ||
| Glutamate (1 mM) | −0.21 ± 0.17 | |
| Kainate (100 μM) | −0.58 ± 0.64 | |
| (1S,3R)-ACPD (100 μM) | −0.57 ± 0.26 | |
| Noradrenaline (100 μM) | 0.63 ± 0.57 | |
| A23187 (10 μM) | 0.30 ± 0.80 | |
| Nicardipine (300 μM) | 3.10 ± 0.23*** | |
| EHNA (300 μM) | 1.44 ± 0.31* | |
| Cilostamide (100 μM) | 1.01 ± 0.08*** | |
| Sildenafil (300 μM) | 19.9 ± 0.34*** | |
The cGMP level remaining in control cells (see Fig 1a) in each experiment has been subtracted (n = 3–6). The mean residual cGMP in control cells was 6.02 ± 1.33 pmol cGMP/106 cells (n = 6) after 2 min and 2.99 ± 0.26 pmol cGMP/106 cells (n = 8) after 5 min. Significance was determined by Student's unpairedt test (* P < 0.05; *** P < 0.001).