Drugs | Kd1-a | Kd |
---|---|---|
Vinblastine | 23.5 ± 7.8 nM | 37.0 ± 10 nM1-b |
36.0 ± 5 nM1-c | ||
Verapamil | 54.2 ± 4.6 μM | 0.45 ± 0.05 μM1-b |
Doxorubicin | 15.0 ± 3.2 μM1-d | 31 ± 7.3 μM1-b |
Cyclosporin A | 62.5 ± 5.6 nM1-e | 18 ± 3.6 nM1-b |
97.9 ± 19.4 nM1-d |
↵1-a These values were measured in the present work by frontal affinity chromatography with immobilized Pgp-IAM.
↵1-b These values are from Ferry et al. (1995).
↵1-c This value is from Callaghan et al. (1997).
↵1-d These values were obtained by displacing [3H]vinblastine (see Experimental Procedures).
↵1-e This value was measured when 3 mM ATP was in the running buffer.