Retention volumes of [3H]vinblastine and [3H]cyclosporin A were obtained when 1) no ATP was present in the running buffer, 2) 3 mM ATP was added in the running buffer, 3) 50 nM cold vinblastine was supplemented in the sample (no ATP in the buffer), and 4) 100 nM cold vinblastine was in the sample (no ATP in the buffer)
| Drugs | Retention Volume (ml) at | |||
|---|---|---|---|---|
| No ATP | 3 mM ATP | 50 nM Vinblastine (No ATP) | 100 nM Vinblastine (No ATP) | |
| [3H]Vinblastine | 32.1 | 8.4 | 11.0 | 9.5 |
| [3H]Verapamil | 34.2 | 5.9 | 34.1 | 34.0 |
| [3H]Cyclosporin A | 7.8 | 17.5 | 15.7 (15.4)2-a | 18.8 |
↵2-a 15.7 ml was measured at the condition of no ATP present in the running buffer, and 15.4 ml was obtained when 3 mM ATP was in the running buffer.