[5-3H]dUrd Incubation time | Ost TK−/ HSV-1 TK(WT)+ | Ost TK−/ HSV-1 TK(Q125N)+ |
---|---|---|
1 h | >100 μM | 9.8 ± 5.1 μM |
2 h | >100 μM | 3.3 ± 2.2 μM |
The ability of BVDU to inhibit thymidylate synthase in Ost TK−/HSV-1 TK(WT)+ and Ost TK−/HSV-1 TK(Q125N)+ cells was measured by the evaluation of tritium release from [5-3H]dUrd after 1 and 2 h.