Inhibitor | Km | Vmax | |
---|---|---|---|
μM | pmol/oocyte/30 min | ||
Inosine | No inhibitor | 15.2 ± 5.3 | 3.19 ± 0.42 |
40 μM thymidine | 35.7 ± 11.5 | 3.20 ± 0.50 | |
Thymidine | No inhibitor | 11.0 ± 2.4 | 3.85 ± 0.26 |
40 μM inosine | 61.4 ± 9.6 | 2.88 ± 0.27 |
CeCNT3-mediated uptake of inosine and thymidine were measured in the absence and in the presence of inhibitors as described underMaterials and Methods. Uptake values are expressed as mean ± S.E. For inosine, thymidine was used as inhibitor; and for thymidine, inosine was used as inhibitor. This experiment was repeated several times and K m and V maxvalues from one representative experiment are given.