Nucleoside uptake of wild-type, Y332F, and T327S/V328L
| Nucleosides | Uptake | ||
|---|---|---|---|
| Wild-type | Y332F | T327S/V328L | |
| pmol/oocyte/30 min | |||
| Uridine | 6.39 ± 0.81 | 4.06 ± 0.58 | 0.72 ± 0.08 |
| Cytidine | 0.46 ± 0.06 | 0.08 ± 0.01 | 0.07 ± 0.01 |
| Thymidine | 8.33 ± 1.15 | 2.98 ± 0.39 | 0.39 ± 0.05 |
| Inosine | 5.62 ± 0.70 | 4.87 ± 0.97 | 0.83 ± 0.09 |
| Guanosine | 7.08 ± 0.88 | 4.44 ± 0.53 | 0.84 ± 0.10 |
| Adenosine | 6.69 ± 0.89 | 3.82 ± 0.63 | 0.99 ± 0.12 |
| Inosine | |||
| K m (μM) | 27.5 ± 3.7 | 102.6 ± 16.5 | |
| V max (pmol/oocyte/30 min) | 39.2 ± 1.7 | 60.6 ± 4.0 | |
| Thymidine | |||
| K m(μM) | 10.6 ± 2.0 | 31.6 ± 5.6 | |
| V max (pmol/oocyte/30 min) | 21.5 ± 0.9 | 19.1 ± 1.1 | |
Wild-type, Y332F, and T327S/V328L mediated uptakes of nucleosides were measured as described under Materials and Methods. Uptake values are expressed as mean ± S.E. These experiments were repeated several times and values for uptake K m, andV max from one representative experiment are given.