Table 2

NB1011 or raltitrexed cytotoxicity and level of TS expression

Cell type	TS protein level	IC₅₀^2-a
Cell type	TS protein level	NB1011	Raltitrexed
		μM	nM
CCD18co	100 ± 22	453 ± 99	20.2 ± 4.6
WI38	150 ± 20	335 ± 5	2.6 ± 0.3
Det551	177 ± 24	262 ± 44	2.6 ± 0.3
H630P	287 ± 35	193 ± 21	6.2 ± 1.2
H630TDX	671 ± 16	0.2 ± 0.1	>1000
H630R10	2405 ± 324	0.5 ± 0.3	330 ± 172
MCF7P	178 ± 45	205 ± 4	1.8 ± 0.1
MCF7TDX	1865 ± 78	0.9 ± 0.5	>1000
MCF7TDX/NB1011^2-b	576 ± 54	278 ± 104	21 ± 15

TS levels were determined by using Western blot analysis; the quantified expression levels were given as values relative to that of cell strain CCD18co (as values of 100). Values are given as mean ± S.E.M.

↵2-a Dialyzed FBS was used.
↵2-b MCF7/TDX/NB1011 is an uncloned cell population selected from MCF7TDX in the presence of 50 μM NB1011.