Effects of ionophores, NEM, and DCCD on PPT1-mediated pentamidine uptake
| Test Compound (μM) | Pentamidine Uptake (% of Control) | Protonmotive Force (mV)2-a |
|---|---|---|
| Control | 100 | −80.8 ± 2.2 |
| CCCP (5) | 56.4 ± 2.2 | −36.0 ± 4.2 |
| CCCP (10) | 29.6 ± 0.6 | −22.8 ± 3.7 |
| Nigericin (5) | 86.2 ± 1.2 | −64.2 ± 4.9 |
| Nigericin (10) | 68.7 ± 2.0 | −40.5 ± 4.8 |
| Nigericin (20) | 44.0 ± 0.8 | −29.1 ± 4.8 |
| Gramicidin (1) | 18.0 ± 3.7 | −20.1 ± 5.6 |
| DCCD (10) | 102 ± 5 | −73.4 ± 3.2 |
| DCCD (100) | 30.8 ± 3.4 | −19.8 ± 8.2 |
| NEM (1000) | 41.7 ± 0.69 | −26.8 ± 3.6 |
Procyclic trypanosomes were incubated with 100 nM [3H]pentamidine as described under Experimental Procedures. Preincubation with inhibitors was 3 min. These data are the average ± S.E. of three independent expts., each performed in quadruplicate and using identical incubations with the same amount of solvent (1% EtOH) as a control.
↵2-a These values were obtained from De Koning et al. (1998).