Table 1

Oligonucleotides used.

OligonucleotideSequence
1 5′-GGAGGCCAGGATAACTGTAG-3′
2 5′-GAGTGGATGTGGTAGCAGA-3′
3 5′-CATGCTGCAGATATGGCTTATTGTGCCGAGTCCCA-3′
4 5′-TGGGACTCGGCACAATAAGCCATATCTGCAGCATG-3′
5 5′-CATGCTGCAGATATGCTTTATGCTGCCGAGTCCCA-3′
6 5′-TGGGACTCGGCAGCATAAAGCATATCTGCAGCATG-3′
7 5′-CATGCTGCAGATATGCTTTATTGTGCCGCTTCCCA-3′
8 5′-TGGGAAGCGGCACAATAAAGCATATCTGCAGCATG-3′
9 5′-ATGCTTCACTGCGCAGCATCCCAC-3′
10 5′-GTGGGATGCTGCGCAGTGAAGCAT-3′
11 5′-CTGGCAATGAATTTCCAAGGGAGG-3′
12 5′-GGTGGAAAGAATCCTTACTCGGGG-3′
13 5′-GCTCTAGAGGCACCATGAGCAGCGGCGCCAACATC-3′
14 5′-GCTCTAGACTACAGGAATCCGCTGGGTGTGAT-3′
15 5′-CCACACCTGTCACTGACAAAA-3′
16 5′-TCAATGAGGCTGTCTGTGATG-3′
17 5′-ACCAGGGCTGCCTTCTCTTGTGACAAAGTG-3′
18 5′-TGAGGTCCACCACCCTGTTGCTGTAGCCAT-3′

The underlined nucleotide doublets denote the mutated bases introduced to generate the alanine-substituted constructs