Effect of SBCE mutagenesis on TGF-β1 responsiveness.
| Relevant Motive | Relative TGF-β1 Responsiveness | |
|---|---|---|
| AhRΔ(−2510) | 5′-CAGTGTCTGGTATATTAT-3′ | 11-a |
| SBCE-1Δ(−2510) | 5′-CAGTTTTTGGTATATTAT-3′ | 0.53 ± 0.08 |
| SBCE-2Δ(−2510) | 5′-CAGTGACCGGTATATTAT-3′ | 0.58 ± 0.07 |
| SBCE-FRΔ(−2510) | 5′-CAGTGTCTGGTAGCGCAT-3′ | 0.78 ± 0.15 |
HepG2 cells were transiently transfected with 1 μg of the indicated constructs and treated with 100 pM TGF-β for 40 h. Promoter activity was determined as described under Experimental Procedures (mean ± S.D.; n = 3).
The core motive is underlined. Mutated bases are indicated in bold letters.
↵1-a The relative luciferase activity in untreated HepG2 cells transfected with AhRΔ(−2510) was 0.4 to 0.5.