Effect of preincubation with salicylate on AA toxicity in HepG2 cells.
| Addition | Percent Toxicity | |||
|---|---|---|---|---|
| A | B | C | D | |
| E47 (buffer) | E47 (Sal) | C34 (buffer) | C34 (Sal) | |
| None | 2.8 ± 2.1 | 5.8 ± 2.5 | 9.5 ± 3.0 | 9.0 ± 4.2 |
| Salicylate (5 mM) | 5.2 ± 3.0 | 5.5 ± 1.9 | 11.8 ± 1.5 | 6.3 ± 1.0 |
| Salicylate (10 mM) | 3.0 ± 0.0 | 4.3 ± 2.6 | 10.8 ± 2.9 | 6.5 ± 2.4 |
| AA (30 μM) | 21.3 ± 4.61-150 | 25.0 ± 8.41-150 | 12.0 ± 2.7 | 11.8 ± 1.0 |
| AA (60 μM) | 37.0 ± 5.11-150 | 42.0 ± 4.41-150 | 12.3 ± 2.9 | 16.5 ± 1.3 |
| AA 30 μM + Sal (5 mM) | 43.5 ± 9.01-160 | 53.3 ± 3.51-160 | 7.8 ± 3.0 | 11.8 ± 1.0 |
| AA 60 μM + Sal (5 mM) | 60.8 ± 2.21-160 | 65.5 ± 3.11-160 | 16.0 ± 6.2 | 8.1 ± 4.0 |
| AA 30 μM + Sal (10 mM) | 61.5 ± 7.41-160 | 68.3 ± 6.21-160 | 11.0 ± 4.5 | 11.0 ± 5.0 |
| AA 60 μM + Sal (10 mM) | 75.0 ± 10.01-160 | 82.5 ± 6.51-160 | 10.5 ± 5.3 | 13.3 ± 0.5 |
E47 cells (A, B) or C34 cells (C, D) were preincubated with buffer (A, C) or with 10 mM sodium salicylate (B, D) for 24 hours. The medium was removed, the cells washed, and the indicated additions were made to all cells, followed by viability assays (Trypan Blue uptake) after 24 h of incubation.