Internal primers used for generating mutations in RFC1
| Mutation | Primers (upstream/downstream) |
|---|---|
| R26L | 5′-GAGATAGAACACCAGGCATAGCCAGGATTTCAGTTCGTG-3′/ |
| 5′-CACGAACTGAAATCCTGGCTATGCCTGGTGTTCTATCTC-3′ | |
| R42L | 5′-CTTCGGCTTCATGGCCCAGTTGCTTCCTGGGGAAAGCTTCATCAC-3′/ |
| 5′-GTGATGAAGCTTTCCCCAGGAAGCAACTGGGCCATGAAGCCGAAG-3′ | |
| R131L | 5′-GTCACCATGGCGGCCCTCATCGCCTACTCCTCC-3′/ |
| 5′-GGAGGAGTAGGCGATGAGGGCCGCCATGGTGAC-3′ | |
| R155L | 5′-ATGGCCAGCTACTCACTGGCGGCAGTACTGCTG-3′/ |
| 5′-CAGCAGTACTGCCGCCAGTGAGTAGCTGGCCATG-3′ | |
| K332L | 5′-CGCTGGACTCTGTGGTCCCTGCTGGTCATCGCAGGTGTG-3′/ |
| 5′-CACACCTGCGATGACCAGCAGGGACCACAGAGTCCAGCG-3′ | |
| R366L | 5′-ACCTTTGTGCTTTTCCTTGGGGCCTACCAGTTC-3′/ |
| 5′-GAACTGGTAGGCCCCAAGGAAAAGCACAAAGG-3′ | |
| K404L | 5′-CTTTCCTAGCTACTGCGCTTCTGACCTGTATCACACTTGTG-3′/ |
| 5′-CACAAGTGTGATACAGGTCAGAAGCGCAGTAGCTAGGAAAG-3′ |
Underlined nucleotides represent the codons that were changed to introduce a corresponding mutation.