Kinetic parameters for MTX influx
| Cell line | Kt | Vmax |
|---|---|---|
| μM | nmol/g of dry wt/min | |
| L1210 | 7.5 ± 1.1 | 10.3 ± 1.1 |
| R26L | 5.2 ± 0.43-a | 13.9 ± 1.6 |
| R42L | 8.3 ± 0.9 | 9.4 ± 3.0 |
| K332L | 10.6 ± 1.33-b | 8.2 ± 1.7 |
| K404L | 10.1 ± 0.43-a | 9.1 ± 1.0 |
K t and V max values are the average ± SEM from three separate experiments determined from nonlinear regression to the Michaelis-Menten equation. Statistical analyses were performed using the Wilcoxon rank sum test, SAS system, version 6.12 (SAS Institute Inc., Cary, NC).