Substrate | L1210 | K404L | Fold difference |
---|---|---|---|
μM | |||
5-Methyl-THF | 2.83 ± 0.61 | 29.68 ± 7.53 | 10.9 |
5-Formyl-THF | 7.33 ± 2.03 | 29.26 ± 4.63 | 4.1 |
Folic acid | 186.7 ± 31.8 | 206.7 ± 29.6 | 1.1 |
Data are the mean ± SEM from three experiments determined from Dixon analyses at 1 and 2 μM [MTX]e.