EC50 values (μM) for GABA- and muscimol-stimulated36Cl influx are shown for GABAA receptors of composition α1-5β3γ2s, stably expressed in Ltk− cells
| Subunit | EC50 | ||
|---|---|---|---|
| 36Cl Flux in Ltk− | Patch-Clamp in Ltk− | ||
| Muscimol | GABA | GABA | |
| μM | |||
| α1 | 0.54 ± 0.06 (17) | 3.9 ± 0.5 (13) | 0.86 ± 0.06 (8) |
| α2 | 0.27 ± 0.04 (14) | 1.6 ± 0.4 (12) | 1.1 ± 0.07 (8) |
| α3 | 2.0 ± 0.23 (17) | 10 ± 0.7 (12) | 15 ± 0.7 (8) |
| α4 | 2.8 ± 0.5 (9) | 6.4 ± 0.6 (17) | 2.6 ± 0.05 (8) |
| α5 | 0.36 ± 0.03 (16) | 1.6 ± 0.3 (17) | 2.8 ± 0.3 (8) |
Electrophysiological responses to GABA in Ltk− cells, measured using a whole-cell patch-clamp technique, are also shown for comparison. Data are presented as arithmetic mean ± S.E.M. with the number of determinations in parentheses.