Increase in Cell Surface Receptors | Increase in Total Receptors | % Receptors at the Cell Surface | |
---|---|---|---|
− Pertussis toxin | 0 | 0 | 48.1 ± 4.7% |
+ Pertussis toxin | 21.0 ± 3.0% | 19.1 ± 12.8% | 46.3 ± 3.0% |
Cell surface and total receptor number were measured using [3H]NMS and [3H]QNB respectively, in the absence or presence of pertussis toxin pretreatment as described underExperimental Procedures. The percentage of receptors at the cell surface was determined by the ratio of bound [3H]NMS to bound [3H]QNB (% = [3H]NMS/[3H]QNB × 100). Values are expressed as mean ± S.E.M.