Quantitative evaluation of the differentiation marker gene expression
| Control | VPA | RA | |||
|---|---|---|---|---|---|
| 1 d | 3 d | 1 d | 3 d | ||
| Collagen IV | 1 | ≤1 | ≤1 | 1.4 | 3.4 |
| Keratin 18 | 1 | 2.9 | 3.2 | 1.7 | 1.7 |
| Laminin β1 | 1 | 1.2 | 1.1 | 1.7 | 3.9 |
Northern hybridization signals were quantitatively evaluated by PhosphorImager analysis relative to the signal on the gapdhblot. Values of the experiment in Fig. 2 are shown and similar results were obtained in two additional experiments.