Target Region/Type | Sequence | Tm |
---|---|---|
°C | ||
Antisense Oligodeoxynucleotides | ||
14-233/5′-UTR | 5′-GsAsGTsCsACsAAGGAsAAGAAGsGsG-3′ | 50.0 |
80-499/START | 5′-AsGsGAsTGTsCGCsGGAAAGsTCsAsT-3′ | 55.7 |
834-1853/CDR | 5′-TsGsTGCsAGTsGAGGGCsTAGAsGsG-3′ | 55.0 |
Control Oligodeoxynucleotides | ||
Random | 5′-TsGsAAsGGCsGACsGAGsAAGGTsGsT-3′ | 56.8 |
Random | 5′-AsTsCGTsCAATsACsTATsCAATsCsG-3′ | 43.8 |
14-233/scrambled | 5′-GsAsGAGAAsAGGAGsCsGAGAsCsAsT-3′ | 49.8 |
80-499/scrambled | 5′-AsGsCAATsGGCsAGGATsACsGGsTsT-3′ | 55.7 |
14-233/mismatched | 5′-GsAsGTsCsACsAAGAsGAAGAAGsGsG-3′ | 47.4 |
80-499/mismatched | 5′-AsGsGAsTGTsCGGsCGAAAGsTCsAsT-3′ | 55.7 |
Melting temperature was calculated according to the nearest neighbor method at 50 mmol salt concentration (Breslauer et al., 1986) with a fully complemented opposite strand and disregarding the destabilization effect of phosphorothioate substituents. Subscript “s” in the base sequence denotes phosphorothioate linkage.