Table 1

Oligodeoxynucleotides

Target Region/Type	Sequence	T_m
		°C
Antisense Oligodeoxynucleotides
14-233/5′-UTR	`5′-G_sA_sGT_sC_sAC_sAAGGA_sAAGAAG_sG_sG-3′`	50.0
80-499/START	`5′-A_sG_sGA_sTGT_sCGC_sGGAAAG_sTC_sA_sT-3′`	55.7
834-1853/CDR	`5′-T_sG_sTGC_sAGT_sGAGGGC_sTAGA_sG_sG-3′`	55.0
Control Oligodeoxynucleotides
Random	`5′-T_sG_sAA_sGGC_sGAC_sGAG_sAAGGT_sG_sT-3′`	56.8
Random	`5′-A_sT_sCGT_sCAAT_sAC_sTAT_sCAAT_sC_sG-3′`	43.8
14-233/scrambled	`5′-G_sA_sGAGAA_sAGGAG_sC_sGAGA_sC_sA_sT-3′`	49.8
80-499/scrambled	`5′-A_sG_sCAAT_sGGC_sAGGAT_sAC_sGG_sT_sT-3′`	55.7
14-233/mismatched	`5′-G_sA_sGT_sC_sAC_sAAGA_sGAAGAAG_sG_sG-3′`	47.4
80-499/mismatched	`5′-A_sG_sGA_sTGT_sCGG_sCGAAAG_sTC_sA_sT-3′`	55.7

Melting temperature was calculated according to the nearest neighbor method at 50 mmol salt concentration (Breslauer et al., 1986) with a fully complemented opposite strand and disregarding the destabilization effect of phosphorothioate substituents. Subscript “s” in the base sequence denotes phosphorothioate linkage.