Table 1

Uptake of various ³H- or ¹⁴C-labeled compounds by hOAT3-expressing oocytes

Tracer	Chemical Concentration Used	Control	hOAT3	p Value
[³H]DHEA sulfate	50 nM	1.7 ± 0.1	33.7 ± 11.1	<0.01
[³H]Estrone sulfate	50 nM∼20 μM	4.5 ± 0.2	23.9 ± 3.0^a	<0.01
[³H]Estradiol glucuronide	50 nM	8.2 ± 0.5	36.2 ± 6.1	<0.01
[¹⁴C]Glutarate	10 μM	2.9 ± 0.2	5.4 ± 1.4	<0.01
[³H]PGE₂	1 μM	0.2 ± 0.1	0.8 ± 0.1	<0.01
[³H]Taurocholate	100 nM	94.8 ± 10.7	275.6 ± 37.7^b	<0.01
[³H]Methotrexate	50 nM∼100 μM	7.9 ± 1.7	17.7 ± 3.4^b	<0.01
[³H]Cimetidine	50 nM∼100 μM	7.1 ± 0.9	82.8 ± 6.0	<0.01
[¹⁴C]p-Aminohippurate	10 μM∼300 μM	0.5 ± 0.1	9.2 ± 1.3^c	<0.01
[³H]Ochratoxin A	50 nM	8.8 ± 0.8	30.8 ± 3.0	<0.01
[³H]cAMP	100 nM	98.9 ± 5.9	140.1 ± 7.7	<0.05
[¹⁴C]Salicylate	100 nM	15.7 ± 0.4	19.5 ± 1.0	<0.05
[¹⁴C]Uric acid	10 μM	0.6 ± 0.2	1.4 ± 0.4	<0.05
[¹⁴C]Valproic acid	5 μM	5.5 ± 0.2	5.1 ± 0.5	N.S.
[¹⁴C]Succinate	10 μM	5.1 ± 0.5	6.4 ± 0.2	N.S.
[³H]Glucuronate	10 nM	5.1 ± 0.7	4.7 ± 0.4	N.S.
[³H]Ibuprofen	10 nM	21.4 ± 2.1	19.5 ± 1.0	N.S.
[³H]Acyclovir	1 μM	0.2 ± 0.0	0.2 ± 0.0	N.S.
[¹⁴C]TEA	30 μM	2.2 ± 0.5	3.0 ± 0.6	N.S.

The uptake experiments were performed at the concentration indicated for 1 h. Values are mean ± SE of 8∼12-oocyte determinations. The significance between water-injected and hOAT3-cRNA-injected oocytes was determined by unpaired ttest. The chemical concentrations used were^a 50 nM,^b 100 nM and^c 10 μM. The units of measure were femtomoles per oocyte per hour for ³H tracers and picomoles per oocyte per hour for ¹⁴C tracers.

N.S., not significant.