Nephrotoxicant | ||||
---|---|---|---|---|
− | + | |||
Lumen | Cells | Lumen | Cells | |
Amikacin (50 μM) | 2200 ± 200 | 500 ± 70 | 1100 ± 190 | 640 ± 80 |
Gentamicin (10 μM) | 2600 ± 130 | 790 ± 60 | 1500 ± 110 | 780 ± 50 |
Diatrizoate (100 μM) | 2000 ± 130 | 410 ± 50 | 740 ± 90 | 440 ± 60 |
Iohexol (300 μM) | 3000 ± 240 | 820 ± 70 | 2100 ± 270 | 880 ± 100 |
Luminal and cellular fluorescence intensities of control tubules and tubules exposed to various nephrotoxicants. All four nephrotoxic chemicals significantly reduced luminal FL-MTX accumulation (P < 0.01), whereas cellular accumulation was not affected. Values are expressed as mean ± S.E., N = 24 to 45 from two to three fish.