Abs Anti- | Retina Extract | after α6, β3 and β2 |
---|---|---|
α2 | 18 ± 2 | 3.7 ± 0.5 |
α3 | 29 ± 1.4 | 8 ± 0.7 |
α4 | 42 ± 6 | 26 ± 3 |
α5 | 10 ± 3 | 1 ± 0.2 |
α6 | 37 ± 2.3 | 1.5 ± 0.5 |
β2 | 30 ± 4 | 4.3 ± 1.5 |
β3 | 26 ± 3 | 2.6 ± 0.2 |
β4 | 74 ± 4 | 33 ± 3 |
The immunoprecipitation was carried out as described underExperimental Procedures using saturating concentrations of anti-subunit Abs. The results are expressed as the percentages of [3H]Epi labeled receptors, taking the amount of receptor present in the solution before immunoprecipitation as 100%. The percentage of immunoprecipitation was subtracted from the value obtained in control samples containing an identical concentration of normal rabbit IgG. The values are the means ± S.E.M. of five determinations.