Table 3

Characteristics of cell surface and internalized 125I PACAP-27 binding in CHO cells expressing PAC1-null and PAC1-hop1receptors

PAC1-nullPAC1-hop1
ExternalInternalExternalInternal
IC50PACAP-27 (nM)20.8  ± 3.210.8  ± 2.313.1  ± 5.89.2  ± 3.8
B max(pmol/mg protein)4.1  ± 0.57.9  ± 0.96.0  ± 0.54.5  ± 0.4