RD50 values of CsA and VRP for all transfectants
| ActD | COLC | VCR | DAUN | |
|---|---|---|---|---|
| ng/ml | ||||
| CsA | ||||
| WT | 101 ± 8 (1.0) | 70 ± 5 (1.0) | 64 ± 1 (1.0) | 90 ± 9 (1.0) |
| I837L | 88 ± 5 (0.9) | 67 ± 4 (1.0) | 81 ± 4 (1.3) | 195 ± 16 (2.2) |
| N839I | 95 ± 8 (0.9) | 97 ± 3 (1.1) | ||
| I837L/N839I | 234 ± 96 (2.3) | 106 ± 17 (1.7) | 174 ± 22 (1.9) | |
| TM6/TM9 Chimera | 504 ± 19 (5.0) | 883 ± 87 (12.6) | 872 ± 35 (9.7) | 757 ± 79 (8.4) |
| VRP | ||||
| WT | 480 ± 41 (1.0) | 169 ± 69 (1.0) | 429 ± 14 (1.0) | 277 ± 6 (1.0) |
| I837L | 340 ± 39 (0.7) | 189 ± 17 (1.1) | 377 ± 2 (0.9) | 347 ± 10 (1.3) |
| N839I | 403 ± 8 (0.8) | 361 ± 37 (1.3) | ||
| I837L/N839I | 344 ± 12 (0.7) | 429 ± 23 (1.0) | 393 ± 49 (1.4) | |
| TM6/TM9 Chimera | 858 ± 84 (1.8) | >2000 (>12) | 462 ± 20 (1.1) | 1648 ± 92 (6.0) |
The numbers in parentheses represent the ratios of the RD50values normalized to those of the wild-type for each drug. The RD50 values for COLC and VCR could not be reliably obtained with the N839I transfectant or with the I837L/N839I transfectant because of the low resistance conferred by these mutations.
WT, wild-type.