Clone | KD | Bmax |
---|---|---|
nM | fmol/mg of protein | |
P1 | 0.21 ± 0.03 | 175 ± 6 |
P3 | 0.20 ± 0.04 | 186 ± 9 |
GRK3 (Clone 2) | 0.21 ± 0.04 | 213 ± 22 |
GRK6 (Clone 6) | 0.21 ± 0.04 | 196 ± 13 |
GRK3 (Clone 12) | 0.19 ± 0.04 | 191 ± 10 |
GRK6 (Clone 24) | 0.21 ± 0.04 | 194 ± 17 |
B max and K D were determined using nonlinear regression analysis and curve-fitting programs of GraphPad Prism II. Data are expressed as the mean specific binding ± S.E.M. for five or six separate experiments.