Table 1

A list of PCR primers for amplifying the following regions of theCyplal gene and cycling conditions

Amplified Region	Primers		Cycling Conditions
Whole gene	ON31:	`AAA TGT GGA CAC ACG CTG`	94°C × 15 s → 58°C × 30 s
	ON32:	`GGG TGT GAA TGT GGC TTA AC`	→ 68°C × 8 min, 35 cycles
Promoter	ON22:	`TGT AAC TAG AGT GGG AGG TG`	94°C × 15 s → 55°C × 30 s
	ON23:	`GCT CCA AGA ACT ACC ACC`	→ 72°C × 90 s, 30 cycles
cDNA	ON38:	`CGG GAT CCT TAC AGC CCA AGC AGC`	94°C × 15 s → 60°C × 30 s
	ON39:	`GGG GTA CCC AGA GCA CTC TTC AGG AG`	→ 68°C × 2 min, 35 cycles
Genomic region containing insertions	5′P:	`TAA TGG CAA GAG CAT GAC`	94°C × 15 s → 55°C × 30 s
	3′P:	`TCC AGA GCC AGT AAC CTC`	→ 68°C × 45 s, 30 cycles