Binding characteristics of HAhIP-GFP mutant receptors
| HAhIP-GFP (n = 4) | N7-Q7 (n = 4) | N78-Q78 (n = 3) | N7,N78-Q7,Q78 | |
|---|---|---|---|---|
| B max (fmol/mg of protein) | 3.34 ± 0.52 | 0.35 ± 0.031-150 | 0.27 ± 0.031-150 | N.D. |
| K d (nM) | 24.3 ± 3.6 | 21.7 ± 1.7 | 149.1 ± 11.11-150 | N.D. |
Saturation binding of [3H]iloprost was carried out using membranes prepared from each cell line. Data are the mean ± SEM from three to four experiments.
↵1-150 p < 0.05 compared with HAhIP-GFP.
N.D., not detectable.