Comparison of recovery of α3β4 receptor function after 60-min treatments with nicotine or carbachol
| Agonist | Agonist concentration for 60-min treatment | Half-time for recovery | Relative maximum recovery |
|---|---|---|---|
| μM | min | % of non-treated cells | |
| (−)-Nicotine | 100 | 7.0 ± 0.5 | 93 ± 2 |
| (−)-Nicotine | 300 | 12 ± 22-150 | 83 ± 4 |
| Carbachol | 1000 | 3.5 ± 0.5 | 103 ± 3 |
| Carbachol | 3000 | 4.1 ± 0.5 | 98 ± 2 |
Values shown are the mean ± S.E.M. of five independent experiments measured in quadruplicate. See Figure 4 for description of experimental procedure and curve fittings.
↵2-150 P < 0.01 compared with half-time for recovery after 100 μM nicotine or after 1000 or 3000 μM carbachol.