Parameters used in fitting the single-channel data
| BC3H1 | WT | 10′αβ | 6′αδ | |
|---|---|---|---|---|
| αapp(s−1) | 310 | 160 ± 80 | 180 ± 80 | 90 ± 40 |
| α′ (s−1) | 1100 | 2100 ± 250 | 900 ± 100 | 1900 ± 800 |
| f (M−1s−1) | 2.1 × 106 | 1.7 ± 0.3 ×106 | 1.5 ± 0.5 × 106 | 0.6 ± 0.2 × 106 |
| b (s−1) | 2000 | 3600 ± 1000 | 2500 ± 700 | 4400 ± 2400 |
| b/f (mM) | 0.95 | 2.1 | 1.7 | 7.3 |
The best fit values for α′ and b for WT receptors are about 2-fold higher than those reported for the interaction of isoflurane with single channels in BC3H1 cells (Dilger et al., 1992). This difference may be the result of an incomplete correction for missed events in the analysis of the latter data.