Cytotoxicity produced by iron and fatty acids in C34 and E47 cells
| Addition | Viability (%) | |
|---|---|---|
| C34 | E47 | |
| None | 100 ± 7 | 100 ± 8 |
| Fe-NTA 25 μM | 88 ± 5 | 74 ± 41-150 |
| Arachidonic acid 10 μM | 96 ± 7 | 89 ± 10 |
| Docosahexaenoic acid 10 μM | 90 ± 10 | 91 ± 12 |
| Eicosapentaenoic acid 10 μM | 85 ± 8 | 81 ± 12 |
| Linoleic acid 10 μM | 91 ± 5 | 97 ± 10 |
| Fe-NTA 25 μM + arachidonic acid 10 μM | 90 ± 12 | 41 ± 51-150 |
| Fe-NTA 25 μM + docosahexaenoic acid 10 μM | 78 ± 15 | 27 ± 41-150 |
| Fe-NTA 25 μM +eicosapentaenoic acid 10 μM | 94 ± 10 | 51 ± 61-150 |
| Fe-NTA 25 μM + linoleic acid 10 μM | 96 ± 12 | 70 ± 31-150 |
C34 and E47 cells were supplemented with or without 10 μM of the following fatty acids: arachidonic acid, docosahexaenoic acid, eicosapentaenoic acid, and linoleic acid, and incubated for 12 h. After a washing step and 12 h of incubation in MEMexps, the cells were supplemented with or without 25 μM Fe-NTA, and incubated for an additional 24-h period. Viability of the cells was then determined by the MTT assay.
↵1-150 Significantly different (p < 0.05, ANOVA) compared with the viability of the corresponding control without any addition.