Equilibrium and kinetic parameters for pancuronium and (+)-tubocurarine obtained from fitting the data in Figs. 3 and 8
| Pancuronium | (+)-Tubocurarine | |
|---|---|---|
| IC50 (nM) (equilibrium) | 5.5 ± 0.5 (24) | 41 ± 2 (41)1-150 |
| Hill coefficient (equilibrium) | 1.13 ± 0.10 | 0.95 ± 0.05 |
| L1,L2 (nM) (two-site model equilibrium) | 6.7 ± 1.2 | N.A. |
| 36 ± 32 | N.A. | |
| ℓon (s−1M−1) (onset) | (2.7 ± 0.9) × 108(14) | (1.2 ± 0.2) × 108 (11)1-150 |
| ℓoff(s−1) (onset) | 1.9 ± 1.2 (14) | 6.8 ± 2.2 (11)1-150 |
| ℓoff (s−1) (recovery) | 2.1 ± 0.7 (12) | 5.9 ± 1.3 (8)1-150 |
| Residency time (ms) 1/ℓoff | 480 | 170 |
| ℓoff/ℓon (nM) | 7.8 ± 3.7 | 50 ± 15 |
The two binding site model (eq. 2) did not converge for the (+)-tubocurarine data. Residency time and ℓoff/ℓonare calculated using the value of ℓoff determined from recovery experiments. The number of original data points (before averaging by concentration) is given in parentheses.
↵1-150 p < 0.001 for t test comparison of (+)-tubocurarine versus pancuronium. Statistical comparisons were not performed for the Hill coefficient or calculated values (residency time and ℓoff/ℓon).