Table 4

Binding of [3H]tiotidine in cotransfected COS-7 cells

COS-7 transfectionKdBmax
nM fmol/mg
pCEFL-HA-H2r21  ± 611.2  ± 1.1 (3)
+ GRK224  ± 75.2  ± 1.1 (3)*
+GRK325  ± 65.9  ± 1.3 (3)*
+ GRK518  ± 510.5  ± 1.2 (3)
+ GRK619  ± 411.2  ± 0.9 (3)

The K d and B max values were calculated using the equation for one binding site. The table shows the mean ± S.E.M.; number of determinations (n) is in parentheses. The K d and B maxvalues of the pCEFL-HA-H2r and the pCEFL-GRK2 and pCEFL-GRK3 were compared using an unpaired t test and found to be significantly different (* p < 0.001).