COS-7 transfection | K_{d} | B_{max} |
---|---|---|
nM | fmol/mg | |
pCEFL-HA-H2r | 21 ± 6 | 11.2 ± 1.1 (3) |
^{+} GRK2 | 24 ± 7 | 5.2 ± 1.1 (3)^{*} |
^{+}GRK3 | 25 ± 6 | 5.9 ± 1.3 (3)^{*} |
^{+} GRK5 | 18 ± 5 | 10.5 ± 1.2 (3) |
^{+} GRK6 | 19 ± 4 | 11.2 ± 0.9 (3) |
The K _{d} and B _{max} values were calculated using the equation for one binding site. The table shows the mean ± S.E.M.; number of determinations (n) is in parentheses. The K _{d} and B _{max}values of the pCEFL-HA-H2r and the pCEFL-GRK2 and pCEFL-GRK3 were compared using an unpaired t test and found to be significantly different (^{*} p < 0.001).