Desensitization of HA-H2r in COS-7 cells overexpressing GRK
| COS-7 transfection | t1/2 |
|---|---|
| min | |
| pCEFL-HA-H2r | 0.49 ± 0.01 (5) |
| + pCEFL-GRK2 | 0.25 ± 0.05 (3)* |
| + pCEFL-GRK3 | 0.30 ± 0.01 (3)* |
| + pCEFL-GRK5 | 0.45 ± 0.05 (3) |
| +pCEFL-GRK6 | 0.48 ± 0.02 (3) |
The apparent rate of desensitization was calculated from the data in Fig. 4C using the equation for monoexponential decay. The table shows the mean ± S.E.M.; number of determinations (n) is in parentheses. The apparent rate of desensitization of the pCEFL-HA-H2r and the pCEFL-GRK2 and GRK3 were compared using an unpairedt test and found to be significantly different (* p < 0.01).