TNF-α/CHX and STS induce caspase 3-like protease activity in MCF-7/Casp-3/Cytochrome c-EGFP cells
| Treatment | DEVD Cleavage Activity |
|---|---|
| A.U./h/μg protein | |
| Vehicle (PBS) | 0.07 ± 0.04 |
| 10 ng/ml TNF-α + CHX | 6.00 ± 0.341-150 |
| 30 ng/ml TNF-α + CHX | 10.17 ± 1.731-150 |
| 100 ng/ml TNF-α + CHX | 14.40 ± 1.061-150 |
| Vehicle (DMSO) | 0.03 ± 0.03 |
| 0.3 μM STS | 0.21 ± 0.20 |
| 1 μM STS | 3.52 ± 0.681-150 |
| 3 μM STS | 11.86 ± 1.131-150 |
Cultures were treated for 6h with TNF-α/CHX, STS, or respective vehicle. CHX was given at a final concentration of 1 μg/ml. Cleavage of fluorigenic Ac-DEVD-AMC was monitored over 2 h using a fluorescent plate reader. Data are means ± S.E.M. from n = 4 cultures per treatment. Experiments were repeated two times with similar results.
A.U., arbitrary fluorescence units.
↵1-150 , p < 0.05, difference from vehicle-treated control cells (analysis of variance and Tukeytest).